Fig. 4 | Nature Communications

Fig. 4

From: Whole proteome analysis of human tankyrase knockout cells reveals targets of tankyrase-mediated degradation

Fig. 4

Rescue and immunoprecipitation analysis of candidates for the proteomic screen. a Reintroduction of tankyrases into DKO cells induces loss of candidate proteins. Immunoblot analysis of HEK293T DKO cells stably expressing a vector control or Flag-TNKS1 and Flag-TNKS2 (T1T2) with the indicated antibodies. b Coimmunoprecipitation of candidate proteins with tankyrase. Immunoblot analysis of HEK293T cells immunoprecipitated with control or TNKS IgG, stained with amido black (left panel) and probed with the indicated antibodies (middle and right panels)

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