Fig. 2

CLEC-2 deficient platelet exacerbates septic shock and multiple organ failure. Mice were injected with LPS (10 mg/kg) for 8 h and a clinical score was assessed. Blood serum biochemistry b LDH, c albumin, d BUN, e ALT (n ≥ 6 in all groups) were measured. Mean values shown with s.d. error bars. f, g Representative images of glomeruli in paraffin embedded kidney sections of unchallenged f or following 8 h LPS g wild-type (CLEC-2^fl/fl), platelet CLEC-2 deficient (CLEC-2^fl/flPF4cre+), GPVI^−/− and double knock out (CLEC-2^fl/flPF4cre + GPVI^−/−) mice using Masson’s trichrome stain. Scale bar = 20 μm. Arrow indicates the presence of epithelial cells and fibrin in Bowman’s space. h Histopathology score for epithelial cell sloughing in the Bowman’s space used as readout of necrosis/apoptosis and i the absence or presence of thrombi in the glomeruli expressed as means ± s.e.m. of 40 glomeruli from 5 mice in each group. Differences assessed using one-way ANOVA with Tukey’s multiple comparison tests ****p < 0.0001. j Representative images of paraffin embedded kidney cortex sections stained with anti-fibrinogen/fibrin antibody 8 h post LPS. Scale bar = 50 μm. k Representative images of paraffin embedded liver sections stained with H&E. Yellow arrows indicate thrombi bars = 200 μm. l Frozen lung sections stained for leukocytes (CD45) and nuclei (Hoechst 33342), Scale bar = 100 μm, or m CD45 and podoplanin. Scale bar = 50 μm. (n = 6 for all histology images)