Fig. 4

CLEC-2-podoplanin axis regulates the inflammatory reaction following LPS. a–f Mice were injected with LPS (10 mg/kg) for 8 h. Pro-inflammatory cyto-/chemokines a IL-6 b TNF-α, c CCL-2, d CXCL-1, e CXCL-2, and anti-inflammatory cytokine f IL-10 levels (pg/mL) measured in the blood 8 h post LPS from wild-type, platelet CLEC-2 deficient (CLEC-2^fl/flPF4cre+), GPVI^−/− and double knock out (CLEC-2^fl/flPF4cre+GPVI^−/−) mice. Mean data shown ± s.e.m. (n = 7 for each group). Differences assessed using one-way ANOVA with Tukey’s multiple comparison tests. *p < 0.05, **p < 0.01, ***p < 0.001. g–l 2 h post LPS, α-PDPN (100 μg/mouse) or Syrian hamster IgG (IgG control) were injected i.v. into WT mice. Pro- and anti-inflammatory cyto-/chemokines g CCL-2, h CXCL-1, i CXCL-2, j IL-6, k IL-10 and l TNF-α levels (pg/mL) were measured 6 h later (i.e., 8 h post LPS). Differences between IgG control group and CLEC2^fl/flPF4cre+ was assessed using Mann–Whitney U-test (n = 7), NS non-significant, *p < 0.05, **p < 0.01