Fig. 6

Platelet CLEC-2 regulates the inflammatory response during bacterial peritonitis. CLEC2^fl/flPF4cre+ or their littermate control mice following cecal ligation and puncture (CLP) for 24 h (n = 12). Immune cell recruitment to the peritoneal cavity was assessed by flow cytometry. a Percentage and b total number of myeloid cells (CD45+CD11b+) from CD45+ cells in peritoneal lavage fluid (PLF). c Percentage and d total number of neutrophils (CD45+CD11b+Ly6G+) in PLF. e Percentage and f total number of macrophages (CD45+CD11b+F4/80+) in PLF. g Percentage of macrophages expressing podoplanin, h Median fluorescence intensity (MFI) of podoplanin expressed on macrophages. i TNF-α, j IL-6, k IL-10, and l CCL-2 levels detected in the PLF. Data are means ± s.d. (n = 7 mice in CLP groups, n = 4 in sham group). Differences between control and CLEC2^fl/flPF4cre+ was assessed using Mann–Whitney U-test. *p < 0.05, **p < 0.01. ***p < 0.001