Fig. 4

The influence of CKAMP44 on firing rate increases with optic tract stimulation number and frequency. a–c The difference in firing rates of relay neurons between genotypes increases with stimulation frequency and with the number of stimuli in the train. The optic tract was stimulated with 25 Hz (a, wildtype: n = 50, CKAMP44^−/−: n = 67), 37.5 Hz (b, wildtype: n = 49, CKAMP44^−/−: n = 28), and 50 Hz (c, wildtype: n = 45, CKAMP44^−/−: n = 55) and firing rates for different stimulus numbers were calculated by dividing the cumulative action potential number by the respective stimulation duration (mean ± SEM, Mann–Whitney test). d Firing rates are significantly higher in relay neurons of CKAMP44^−/− mice than in those of wildtype mice when stimulating such that the first stimulus was just suprathreshold and elicited an action potential (wildtype: n = 31, CKAMP44^−/−: n = 11, mean ± SEM; responses to 3, 4, and 5 stimuli: Mann–Whitney test, other responses: t-test). *p < 0.05, **p < 0.01, ***p < 0.001