Fig. 5

CKAMP44 decreases peak firing rate of ON and OFF-responses in vivo. a ON-response amplitude is significantly higher in CKAMP44^−/− than in wildtype mice. ON-responses were observed briefly after increasing the light intensity of the monitor (n = 542 dLGN neurons in 6 wildtype and 430 dLGN neurons in 6 CKAMP44^−/− mice; mean ± SEM). b Deletion of CKAMP44 increases also the OFF-response amplitude (mean ± SEM, Mann–Whitney test) (n = 309 dLGN neurons in 6 wildtype and 285 dLGN neurons in 6 CKAMP44^−/− mice). a, b Raw traces of tetrode recordings are shown on the top, raster and peristimulus time histograms in the middle and average ON- and OFF-responses at the bottom. Red bars indicate spikes of dLGN neurons. c Quantification of the peak firing rate and the rate increase for ON- and OFF-responses (box-and-whisker plots showing the median, IQR and 5–95% range, Mann–Whitney test). *p < 0.05, **p < 0.01, ***p < 0.001