Fig. 1
Metabolome functional analysis by multiplexed activity metabolomics (MAM). a High-data-content metabolomic arrays are generated in replicate from a “stimulus” organism via split-flow polarity switching chromatography mass spectrometry. A suspension of disaggregated tissue cells from a “response” organism (human) is added to the metabolomic array. b Flow cytometric cell barcoding and multiplexed immunoassays are used to identify multiple cell type/subtype-specific biological responses to metabolites in the array. Correlation analysis of the resulting bioactivity and UV/ESI/MS(±) data generate putative functional activities for metabolites. An example of MAM with a natural product producing actinomycete and a cell preparation derived from an AML patient including, but not limited to lymphocytes, monocytes, and leukemia blasts, as well as their subtypes is illustrated here
