Fig. 4 | Nature Communications

Fig. 4

From: Discovery of human cell selective effector molecules using single cell multiplexed activity metabolomics

Fig. 4

An optochemical cell selectivity switching natural product in Nocardiopsis revealed by primary cell MAM. The top metabolome row (orange) shows total ion current and extracted ions for ciromicins A and B (m/z = 515, 15, and 16 min) and apoptolidin (m/z = 1129.5, 23.4 min) with their elution times shown in dotted lines. The next four rows show selected bioactivity chromatograms from a single flow experiment, which were generated by adding an aspirated preparation of bone marrow mononuclear cells from an AML patient, barcoding, immunostaining, and debarcoding. The immunostaining panel contained CD45 (leukocyte-common antigen), cCasp3 (cleaved caspase), γH2AX (DNA damage), p-Histone H3 (cell cycle marker upregulated during mitosis), and p-S6 (marker for active translation). The average collected per well was 5400 blasts (minimum 209), 750 monocytes (minimum 19), and 291 lymphocytes (minimum 20). Histograms for each marker for highlighted wells are shown in Supplementary Fig. 6

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