Fig. 5
Photochemical isomers ciromicins A and B selectively target different cell subsets within the heterogeneous mixture of patient biopsy cells. Mass cytometry uses DOTA-chelated metals detected by inductively coupled plasma mass spectrometry (ICP-MS) to eliminate spectral overlap, expanding the feature range to 29 antibody-quantified features per cell. a viSNE maps of 20,000 individual cells from each condition are organized according to differences in their surface marker profiles for each treatment condition. b MEM labels for three blasts subsets and plots of population prevalence with observed prevalence in white and 95% binomial confidence interval represented by a box. c Marker enrichment modeling (MEM) was used to characterize major populations within the samples and highlight differences in marker expression relative to a gated population of phenotypic hematopoietic stem cells (gate 11). Heat maps of hierarchical clustered MEM labels reveal subsets specific differences and cellular identification. Heat maps of median marker expression are shown in Supplementary Fig. 7. Dose–response data against PMBCs from a healthy donor and patient sample 015 are shown in Supplementary Fig. 8
