Fig. 2
From: Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors
Libraries of −10 and −35 sites cover a wide range of expression. a Diagram of the promoters showing essential features. Each promoter contained a proximal operator site for either AraC or LasR, a constant downstream reporter (YFP), and −10 and −35 sites chosen from the lists below. In each list, bases that do not match the consensus sequence are underlined. b Experimentally measured transcription rates of promoters regulated by either AraC (left column) or LasR (right column) with (bottom row) and without (top row) inducer for every combination of the −10 and −35 sites listed in a. c Fold-change induction heat maps for either AraC (left column) or LasR (right column). Experiments were performed with biological triplicates. Experimental data are available in Supplementary Data 1–3
