Fig. 5: Passive tension of cardiomyocytes of WT and PP5 TG mouse hearts
a Representative image of permeabilized cardiomyocyte glued at the ends to a force transducer and micromotor, respectively, and experimental protocol. Bar, 20 µm. b Passive tension (Fpassive) vs. SL curves of permeabilized single PP5 TG and WT cardiomyocytes in relaxing solution, before and after treatment with recombinant PP5c. c Fpassive-SL curves of permeabilized single PP5 TG and WT cardiomyocytes before and after treatment with ERK2. d Fpassive–SL curves of permeabilized single WT cardiomyocytes before and after treatment with ERK2 and additional exposure to PP5c. Data are mean ± s.e.m., n = 5 cells/condition (2 different hearts/condition). Curves are second-order polynomial fits to the means. *p < 0.05, TG vs. WT; #p < 0.05, WT + PP5c vs. WT in b and WT + ERK2 vs. WT in c and d; †p < 0.05, TG + ERK2 vs. TG in c and WT + ERK2 + PP5c vs. WT + ERK2 in d; all by two-tailed Student’s t-test
