Fig. 5

Rapamycin over-rides the effect of miRNA inhibitors. a Representative images of alkaline phosphatase (blue) and Oil Red O (red) staining of hMSCs transfected with miRNA inhibitors with and without 50 nM rapamycin. Scale bar, 100 μm. b Differentiation bias of hMSCs treated with miRNA inhibitors with (grey bars) and without (black bars) 50 nM rapamycin. Data is shown as mean ± SD of the change in proportion of osteoblasts (see Supplementary Fig. 7 for additional donors). Samples were analysed by one-way ANOVA with Tukey post hoc testing. Statistically different samples are denoted by *p < 0.05, **p < 0.01 and ***p < 0.001