Fig. 2
From: Yolk sac macrophage progenitors traffic to the embryo during defined stages of development
Pre-macrophages infiltrate embryonic tissues. a Isolated E10.5 Cx3cr1GFP/+ embryo. b, c Visualization (b) and quantification (c) of CX3CR1+ macrophages in different embryonic regions at indicated time points; * p < 0.05 (one-way ANOVA with Tukey’s multiple comparisons test: head vs. trunk p = 0.0107, head vs. tail p = 0.0121, trunk vs. tail p = 0.9860); graph shows median ± IQR. d Quantification by flow cytometry of CX3CR1 GFP+ cells in the embryonic YS, brain and trunk at indicated time points; (**) p = 0.0017 (two-tailed Mann–Whitney test); median ± IQR. e CX3CR1 GFP+ cells (green) in the embryonic brain at E16.5 additionally stained for the endothelial marker CD31 (red). f Image series extracted from live E10.5 video sequences (Supplementary Movie 6) show a cell attaching to the endothelium in the embryonic head region; direction of flow from top to bottom. Scale bars are 1 mm (a), 100 µm (b, e, f)
