Fig. 4

Intravascular trafficking is independent of MYB and CX₃CR1. a Schematic graph of the Cx₃cr1^GFP/+ Myb^−/− mouse model. b Bright field images of isolated embryos at E16.5 indicating the absence of fetal definitive hematopoiesis (i.e. severe anemia). c, d Visualization (c) and quantification (d) of CX₃CR1+ cells in the YS of Myb^+/+ or Myb^−/− mice at indicated time points; all comparisons of Myb^+/+ vs. Myb^−/− are n.s. (two-tailed t-test: E9.5 p = 0.9818, E10.5 p = 0.8894, E12.5 p = 0.6900, E16.5 p = 0.9543); mean ± standard deviation (SD, error bars). e Quantification of intravascular CX₃CR1+ cells in a Myb^+/+ and Myb^−/− YS on indicated time points; E10.5: n.s. (two-tailed Mann–Whitney: E10.5 p = 0.4537); median ± IQR. f Quantification of CX₃CR1+ cell densities per microscopic field of 400 × 400 µm in embryonic tissues at E10.5; *** p < 0.001 (two-tailed t-test); mean ± SD. g Quantification of intravascular CX₃CR1+ cells in YS of Cx₃cr1^GFP/+ and Cx₃cr1^GFP/GFP E10.5 embryos; n.s. (two-tailed t-test: p = 0.6065); mean ± SD. Scale bar is 100 µm (c)