Fig. 6

Cellular expansion and morphology of CSF1R+ progenitors. a Schematic graph for the Csf1r^MerCreMer:Rosa26^eYFP mouse model with OH-TAM pulse labeling at E7.5 or E8.5 (red arrows) and subsequent analyses at indicated time points (black arrows). b, c Fluorescence pictures of E10.5 YS and embryo after OH-TAM pulse at E7.5 or E8.5 as indicated (b) with corresponding quantifications c; *** p < 0.001 (two-tailed Mann–Whitney test); median ± IQR. d Fluorescent pictures in the YS and embryo at E8.5, E9.0 and E9.5 with OH-TAM injection 24 h earlier. e, f Fluorescence pictures of a E12.5 YS and different embryonic regions after OH-TAM pulse labeling on E8.5 (e) with corresponding quantification of cluster size (f); *** p < 0.001 (Kruskal–Wallis test with Dunn’s multiple comparisons test); median ± IQR. g Quantification of intravascular cells at indicated time points in an average-sized vessel; median ± IQR. h Fluorescence pictures of a E10.25 YS in a Csf1r^MerCreMer:Rosa26^tdTomato (red):Cx₃cr1^GFP/+ (green) mouse model with OH-TAM pulse labeling at E8.5. Additional staining for F4/80 (purple) and CD31 (turquoise). i, j Adult liver sections of Csf1r^MerCreMer:Rosa26^eYFP mice, pulse-labeled at E8.5 (i) with additional stainings for CD31 (red) and DAPI (blue) (j). Scale bars are 100 µm (b, d, e, j), 1 mm (i), 50 µm (h)