Fig. 2

ZIKV infection alters decidua and villous placenta monocyte and CD4+ T-cell populations and activation status. Decidua and villous placental lymphocytes were isolated from ZIKV-infected and control RM and analyzed by flow cytometry for the presence of the cellular activation marker CD169 on a dendritic cells; b monocytes; c monocyte subsets; and d NK cells, or the marker for Ki67 proliferation marker on e CD4+ central memory T cells and f CD4+ effector memory T cells. Cells were stained with a panel of antibodies recognizing HLA-DR, CD14, CD11c, CD123, CD20, CD3, CD16, and CD169 or a secondary panel containing CD3, CD4, CD8β, CD95, CD28, and Ki67. One-way ANOVA was performed with Tukey’s multiple comparison test, bars represent mean and SEM, ***p < 0.001, **p < 0.01, *p < 0.05