Fig. 3
Neural coding in the MOs. a The experimental design. b Cranial window for two-photon imaging. Arrowheads indicate 700 µm rostral and 700 µm lateral from the bregma. Note a bridging vein leading to the superior sagittal sinus, which often runs around the coordinates. c Virally expressed GCaMP6f in the MOs for two-photon calcium imaging during the visually guided eye movement task. d Averaged fluorescence change of two example cells (ROIs shown in c, aligned to the onset of the visual target or to the onset of the eye movement; cyan, the trial condition for ipsiversive eye movement; magenta, for contraversive). e, f Sorted normalized ∆F/F traces for 904 neurons showing significant response either to visual targets or to eye movements. Response was aligned to the onset of the visual target or the movement in the contraversive (e) or ipsiversive (f) trials. g–j Comparison between contraversive and ipsiversive movement trials for visual and motor activity (n = 519 or 471 neurons, showing significant visual or motor activity). i, j A histogram for difference between ipsiversive and contraversive conditions in visual or motor activity. Neurons with a larger response in the contraversive condition (p < 0.05, Mann–Whitney test) were labeled as magenta (n = 135 in visual activity, 122 in motor activity) and neurons with a larger response in the ipsiversive condition in cyan (n = 80, 45). The population preferred for the contraversive condition in visual activity (n = 519, p < 0.05, Wilcoxon signed-rank test) and in motor activity (n = 471, p < 10−12)
