Fig. 1
The dual-input Mother Machine. a Overview of the dual-input Mother Machine (DIMM) design. b Dial-a-wave junction in three different switching states, top: 100% from input 1 (unlabeled) and 0% from input 2 (green), middle: 50% from both inputs, bottom: 0% from input 1 and 100% from input 2. c Phase contrast image of growing Escherichia coli cells in three growth-channels of the DIMM. d A time series of a single growth-channel containing E. coli cells expressing LacZ-GFP from the lac promoter while being exposed to media which alternate between containing glucose and lactose as a carbon source. e Overview of the automated and curation phases of the MoMA analysis pipeline. f Histogram of the fraction of curated frames per single growth-channel time course. g Estimated measurement errors on cell size (blue) and number of GFP molecules (red). Dark blue points indicate the typical range of cell sizes. Error bars show standard errors. The black line shows the fitted function \(1.01{\mathrm{/}}\sqrt {\left( x \right)}\)
