Table 1 Experimental conditions used in this study

From: Monitoring single-cell gene regulation under dynamically controllable conditions with integrated microfluidics and software

Condition

Sequence of growth media

Strain

No GFP

12 h: M9+0.2% glucose

MG1655

12 h: M9+0.2% lactose

Glucose

22 h: M9+0.2% glucose

ASC662

Lactose

22 h: M9+0.2% lactose

ASC662

Switch

4 h: M9+0.2% glucose

ASC662

4 h: M9+0.2% lactose

4 h: M9+0.2% glucose

4 h: M9+0.2% lactose

4 h: M9+0.2% glucose

4 h: M9+0.2% lactose

Switch IPTG

4 h: M9+0.2% glucose

ASC662

4 h: M9+0.2% lactose+500 μm IPTG

4 h: M9+0.2% glucose

4 h: M9+0.2% lactose+500 μm IPTG

  1. Strain MG1655 is a reference K12 strain46, and ASC662 was derived from it by integrating a translational fusion lacZ-gfp in the native lac operon7. Note that for each condition, the first step of its sequence of growth media was preceded by 2 h in the same media (in order to reach growth steady-state under fluorescence illumination conditions) that were discarded from the data analysis
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