Fig. 5 | Nature Communications

Fig. 5

From: Importance of the 1+7 configuration of ribonucleoprotein complexes for influenza A virus genome packaging

Fig. 5

Co-immunoprecipitation of 18S and 28S rRNA with the NP protein. Purified virions were disrupted in buffer containing TritonX-100, incubated with an anti-NP antibody, and precipitated with Protein G beads. a The precipitates were subjected to western blot analysis using an anti-whole virion polyclonal antibody. b–d RNAs were extracted from the precipitate and were subjected to northern blotting by using riboprobes specific for NS vRNA (b), 18S rRNA (c), and 28S rRNA (d). IP immunoprecipitation

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