Fig. 4

Depletion of PHF8 increases the enrichment of H4K20me1 on overexpressed genes in Phf8 deficits. a Amplicons for real-time PCR are illustrated for three direct PHF8 target genes: Kras; Camk2d; Rps6ka1; and Mapk14. The black arrow represents the transcription start site (TSS) of genes, small black squares indicate exon. Short lines below represent the locations of the amplicons by different primers. Scale bar = 1 kb; b Conventional CHIP analysis with PHF8 antibody shows the specific binding of PHF8 on TSS loci of its targeting genes. c The enrichment of H3K9me2 on the PHF8 target genes is not changed by deletion of Phf8. d The enrichment of H4K20me1 on the overexpressed Kras, Camk2d, and Rps6ka1 were enhanced by deletion of Phf8, while the enrichment of H4K20me1 was not changed in the TSS of Mapk14. Real-time PCR was performed on the input and immunoprecipitated genomic fragments. Data are presented as percentage of input. Numbers on the x axes represent the different amplicons. All data are shown as mean ± s.e.m. *p < 0.05; **p < 0.01 and ***p < 0.001