Fig. 1
From: A neural basis for antagonistic control of feeding and compulsive behaviors
LH GABA and glutamate neurons send monosynaptic projections to a common subset of PVH neurons. a Experimental schematic. b Representative image showing ChR2-EYFP LHPdx1 fibers in PVH. c ChR2 expression in LHPdx1 neurons. Coordinates in a, b are anterior–posterior (AP) measurements relative to Bregma. III, 3rd ventricle; AHP, anterior hypothalamic area, posterior; f, fornix; ic, internal capsule; LH, lateral hypothalamus; opt, optic tract; PVH, paraventricular nucleus of the hypothalamus; Scale bar = 300 µm. d Electrophysiology traces from patch-clamp recordings showing optical-evoked inhibitory post-synaptic currents (oIPSCs, top) and optical-evoked excitatory post-synaptic currents (oEPSCs, bottom) in a PVH neuron held at the indicated potentials (V h) following 1-ms blue light pulse to LHPdx1-ChR2 fibers. Currents can be blocked by ionotropic GABA or glutamate receptor blockers, GABAzine and CNQX+APV, respectively. e Effect of co-application of TTX and 4-AP in oIPSCs (top) and oEPSCs (bottom). f Pie chart showing percentage of PVH neurons receiving excitatory and/or inhibitory inputs from LHPdx1-ChR2 neurons, as suggested by electrophysiology current responses to blue light
