Fig. 4
From: A neural basis for antagonistic control of feeding and compulsive behaviors
GABA-A and ionotropic glutamate receptor activation in PVH are required for LHPdx1-ChR2→PVH evoked feeding and grooming, respectively. a Inhibitory (oIPSC, top) and excitatory (oEPSC, bottom) post-synaptic current responses evoked by 1-ms blue light pulse (blue tick) in a PVH neuron of a Pdx1-Cre::γ2 flox/flox mouse (control). b oIPSC (top) and oEPSC (bottom) responses to 1-ms blue light pulse in a PVH neuron of a Pdx1-Cre::Sim1-Cre:: γ2 flox/flox mouse (double-cre knockout). c Quantification of current amplitude in control and double-cre knockout mice. Number of cells showing current response out of total number of cells recorded is shown above bars. Data are presented as mean ± s.e.m. (two-tailed Mann–Whitney test; median for control mice = 101.3, n = 10; median for double-cre knockout mice = 19.8, n = 4; U = 4; *P = 0.0240). d Food intake before (pre-light), during (light-on), and after (post-light) 5 Hz, 100 ms photostimulation of LHPdx1-ChR2→PVH fibers in control (n = 7) and double-cre knockout mice (n = 6). Two-way repeated measures ANOVA followed by Dunnett’s multiple comparisons test. (interaction F [2, 22] = 28.85, P < 0.0001; genotype F [1, 11] = 29.11, P = 0.0002; light F [2, 22] = 28.85, P < 0.0001; subjects [matching] F [11, 22] = 1.01, P = 0.4689. Pdx1-Cre::γ2 flox/flox [pre-light vs. light-on], *** p < 0.0005). e Schematic illustrating custom-made guide cannula allowing for interchangeable fluid and optical delivery to PVH. f Approximate fluid injection location in PVH area is shown by microinjection of blue ink before sacrifice. Scale bar = 500 µm. g Time spent grooming during 5 min of 5 Hz, 10–50 ms photostimulation of LHPdx1-ChR2→PVH following vehicle or D-AP5 + DNQX microinjection to PVH (two-tailed ratio paired t-test; t = 6.862 df = 2; *P = 0.0206; n = 3)
