Fig. 5
From: A neural basis for antagonistic control of feeding and compulsive behaviors
Inhibition of GABAergic LHPdx1→PVH fibers reduces feeding after a fast, and inhibition of glutamatergic fibers reduces water-spray induced grooming. a Experimental schematic. b Experimental protocol used to test fast-refeeding in Pdx1-Cre::Vglut2 flox/flox::eArchT3.0 mice under mock or 556 nm light inhibition. c Time spent refeeding after a fast during mock inhibition trial. d Time spent re-feeding after a fast under + Light trial, whereby 556 nm constant light was applied every other minute during 10 consecutive minutes. e Comparison of cumulative time spent feeding during light-off vs. light-on periods during + Light and Mock inhibition trials. n = 3 animals. Two-way repeated measures ANOVA followed by Sidak’s multiple comparisons test; light group F (1, 2) = 0.01648, P = 0.9096; light period F (1, 2) = 45.77, P = 0.0212; interaction light group X light period F (1, 2) = 35.58, P = 0.0270. Light-off vs. Light-on period (+Light group) *p < 0.05. Data are presented as mean ± s.e.m. f Experimental protocol used to test water spray induced grooming in Pdx1-Cre::Vgat flox/flox::eArchT3.0 mice under mock (447 nm) or 556 nm light inhibition. g Time spent grooming during mock and 556 nm inhibition when no water spray was delivered. h Time spent grooming during mock and 556 nm inhibition when water spray was delivered to induce grooming. i Simultaneous time spent feeding during water spray trials. g–i Two-tailed paired Student's t test; n = 5; g t = 1.935, df = 4, p = 0.1250; h t = 7.779, df = 4, **p = 0.0015; i t = 2.731, df = 4, p = 0.0524 (ns, not significant)
