Fig. 5

Intermittent PTH treatment increases the number of contact. a 3D colocalization analysis of representative images from control mice and 1-w-, 3-w-, and 6-w-PTH-treated Col2.3-ECFP/TRAP-tdTomato mice. Cyan, mOBs expressing Col2.3-ECFP; red, mOCs expressing TRAP-tdTomato. The contact areas were defined as the areas of colocalization of mOBs and mOCs, and are shown in yellow. Scale bar, 100 µm. b The number of mOB–mOC contacts. The duration of a mOB–mOC contact event was defined as the time from initial attachment to the end of mOB–mOC contact. c The numbers of contact events normalized by the surface areas of the mOBs. d The numbers of contact events normalized by the surface areas of the mOCs. b–d The data were collected from seven to eight independent experiments per group (control; n = 7, 1-w-PTH; n = 7, 3-w-PTH; n = 8, 6-w-PTH; n = 7). e The duration of mOB–mOC contact. Data were collected in seven to eight independent experiments performed per group (control; n = 322, 1-w-PTH; n = 375, 3-w-PTH; n = 1,252, 6-w-PTH; n = 1,126). Data are presented as means ± SDs. *p < 0.05; **p < 0.01; ***p < 0.001; NS, not significant (Kruskal–Wallis test)