Fig. 6 | Nature Communications

Fig. 6

From: Direct cell–cell contact between mature osteoblasts and osteoclasts dynamically controls their functions in vivo

Fig. 6

Direct contact attenuates resorbing activity in PTH-treated bone. a–d Representative MIP images of the bone-resorptive activities of control and 1-w-, 3-w-, and 6-w-PTH-treated Col2.3-ECFP/TRAP-tdTomato mice using a pH-sensing chemical probe, pHocas-3. The binarized mOC areas and the pHocas-3 signals are indicated in the bottom panels. Green, fluorescent signals from pHocas-3; Cyan, mOBs expressing Col2.3-ECFP; red, mOCs expressing TRAP-tdTomato; blue, bone tissues (SHG). Scale bar, 50 µm. e BRI time courses for the visual fields shown in a–d. f Correlations between CMI and the mean BRI values over 4 h; n = 12, collected from 8 to 10 mice per group. Data are presented as means ± SDs. ***p < 0.001 (Spearman’s correlation coefficient, r = −0.5717; tests for no correlation, p = 0.0003.)

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