Fig. 2
Biotransformation of polyunsaturated fatty acids to hepoxilins and trioxilins in Escherichia coli. Different combinations of M. xanthus genes 12-lipoxygenase (12-LOX) or 11-LOX and epoxide hydrolase (EH) were expressed in E. coli strains. HXs hepoxilins, TrXs trioxilins. a Biotransformation of ARA to HXB3 by recombinant E. coli expressing 12-LOX. b Biotransformation of EPA to HXB4 by recombinant E. coli expressing 12-LOX. c Biotransformation of DHA to HXB5 by recombinant E. coli expressing 12-LOX. d Biotransformation of ARA to HXD3 by recombinant E. coli expressing 11-LOX. e Biotransformation of ARA to TrXB3 by recombinant E. coli expressing 12-LOX and EH. f Biotransformation of EPA to TrXB4 by recombinant E. coli expressing 12-LOX and EH. g Biotransformation of DHA to TrXB5 by recombinant E. coli expressing 12-LOX and EH. h Biotransformation of ARA to TrXD3 by recombinant E. coli expressing 11-LOX and EH. The time-course reactions were performed in 50 mm 4-(2-hydroxyethyl)piperazinyl-1-propanesulphonic acid (EPPS) (pH 8.5) buffer containing 1 mm PUFA, 7.2 g L−1 cells for ARA or 14.4 g L−1 cells for EPA or DHA at 30 °C for 90−120 min. Data represent the means of three separate experiments, and error bars represent the standard deviations. The symbols indicate PUFA (circles), hydroperoxy fatty acid (HPFA) (squares), hydroxy fatty acid (HFA) (upward-pointing triangles), HX (diamonds) and TrX (downward-pointing triangles)
