Fig. 3
Transcriptional activity of peroxisome proliferator-activated receptor gamma for hepoxilins and trioxilins. HEK-293 cells were cultured in a 24-well plate (1.0×105 cells per well). After 24 h, cells were transfected with plasmids expressing peroxisome proliferator-activated receptor gamma (PPARγ), PPAR response element (PPRE)×3-thymidine kinase-luciferase reporter constructs, and the Renilla luciferase control vector pRL. After another 24 h, cells were treated with HXs, TrXs and/or 3 μm troglitazone (TRO) for 24 h. Cells were harvested, and the transcriptional activity of PPARγ was determined by a luciferase assay. HXs hepoxilins, TrXs trioxilins. a HXB3. b HXB4. c HXB5. d HXD3. e TrXB3. f TrXB4. g TrXB5. h TrXD3. Data represent the means of three separate experiments, and error bars represent the standard deviations. p-value are based on t-test. *p < 0.05, **p < 0.01. n.s. indicates not significant
