Fig. 5
Degradation of complex RNA structures by Cg-mtEXO and Cg-Dss1. a RNA degradation assay with structured RNA substrates (listed in Supplementary Fig. 10) for full-length Cg-mtEXO or Cg-Dss1. Degradation products were analyzed by 18% denaturing TBE-urea PAGE. b Plot of R36 RNA degradation kinetics (see Supplementary Fig. 9d for images of PAGE analysis) by fl-Cg-mtEXO or fl-Cg-Dss1 in the presence of ATP (mean ± s.e.m. from three experiments). The final degradation products are shown as the sum of 2–5 nt RNA fragments. c Plot of L1 RNA degradation kinetics based on results shown in a by fl-Cg-mtEXO or fl-Cg-Dss1 in the presence of ATP (mean ± s.e.m. from three experiments). The final degradation products are shown as the sum of 2–6 nt RNA fragments
