Fig. 7
Cavin-1 drives SOCS3 localisation to the plasma membrane. a WT (cavin-1+/+) and cavin-1−/− MEFs transiently expressing SOCS3-GFP (green) were stained with DAPI prior to being fixed, solubilised, and stained with anti-cavin-1 antibody (red) before mounting for imaging by confocal microscopy. Areas of red and green overlap are yellow. Scale bar = 10 μm. b Pearson's correlation coefficient value (r2) measured from the intensity values located within the rectangular region on the plasma membrane superimposed on the merged cavin-1/SOCS3-GFP image from WT MEFs. c Cavin-1−/− MEFs transiently co-expressing SOCS3-GFP (green) and cavin-1-mCh (red) were stained with Hoechst 33342 prior to being fixed for imaging by confocal microscopy. Areas of red and green overlap are yellow. Scale bar = 10 μm. d WT, SOCS3−/−, and cavin-1−/− MEFs were pre-incubated with Fsk (50 μM) for 5 h to induce SOCS3 prior to subcellular fractionation and analysis by SDS-PAGE and immunoblotting with the indicated antibodies
