Fig. 3
From: HoxC5 and miR-615-3p target newly evolved genomic regions to repress hTERT and inhibit tumorigenesis
HoxC5 inhibits hTERT expression. a A schematic representation of mir-615-3p genomic localization in the intron of HOXC5. b, c Relative hTERT mRNA expression and telomerase activity in HeLa, PC-3, U251, or BT549 cells transduced with lentivirus overexpressing Flag-tagged HOXC5. The telomerase activity in cells transiently transfected with control GFP vector is set as (1). d Telomere length in HeLa, PC-3, U251, or BT549 cells transduced with lentivirus overexpressing GFP or Flag-tagged HOXC5. PD: population doubling. e The expression of endogenous HOXC5 mRNA in HeLa cells expressing control shRNA or two independent anti-HOXC5 shRNAs. f Relative hTERT mRNA expression in HeLa cells expressing control shRNA or two independent anti-HOXC5 shRNAs. g Telomere length in HeLa cells transduced with lentivirus overexpressing control shRNA or two independent anti-HOXC5 shRNAs. Significance was determined by t test. *P < 0.05. PD population doubling
