Fig. 5
From: HoxC5 and miR-615-3p target newly evolved genomic regions to repress hTERT and inhibit tumorigenesis
HoxC5 recruits Pbx4 and Meis3 to suppress hTERT expression in HeLa cells. a Schematic representation of HOXC5 function domains with its HX and HD motifs highlighted in black. The mutations introduced in HX or HD motif are illustrated, respectively. b, c Relative hTERT mRNA expression and telomerase activity in HeLa cells transduced with lentivirus overexpressing Flag-tagged GFP, wild-type (WT), or mutants (M1 or M2) HOXC5. d Western blotting shows the expression of Flag-tagged GFP, wild-type (WT) and mutants (M1 or M2) HoxC5 expressed in HeLa cells. e Immunoprecipitation of V5-tagged HoxC5 resulted in specific co-immunoprecipitation of Flag-tagged Pbx4, but not Flag-tagged Pbx1, 2 or 3 in HeLa cells. f The purified recombinant 6xHis-tagged HoxC5 proteins from bacteria interact with the in vitro translated and 35S-Methionine labeled Pbx4 proteins, but not Pbx1, 2, or 3. g Immunoprecipitation of Flag-tagged HoxC5 resulted in co-immunoprecipitation of V5-tagged Meis3, but not V5-tagged Meis1 or 2 in HeLa cells. h, Transient transfection of plasmids co-expressing PBX4 or MEIS3 with HOXC5 results in synergistic suppression of hTERT mRNA expression in HeLa cells. Significance was determined by t test. *P < 0.05
