Fig. 3
From: Pixelated spatial gene expression analysis from tissue
Tissue pixelation and Bulk picoliter reagent loading characterization. a–d SEM characterization after tissue pixelation. Tissue partitioning and division into small pixels can be clearly visualized as tissue is seen inside the wells. The blue box in a is shown in b and the blue box in b is shown in c, d (scale bar: a 200 µm; b 50 µm; c, d 20 µm). e, f DAPI-fluorescence imaging of the same pixelated tissue showing nuclei inside the well boundaries. f shows the region in yellow box in e (scale bar: e 200 µm; f 50 µm). g, h Characterization after bulk picoliter reagent loading in tissue-loaded wells. Fluorescent rhodamine dye was filled in the wells for characterization of cross-over across wells. g shows the low-magnification image of dye-filled tissue (*) and no tissue (**) regions and h shows the high magnification image of a dye-filled region (shown in yellow box in g) with tissue. Well edges are seen as dark lines showing that they are above the fluid level and there is no overflow between adjacent wells. Partially filled wells indicated by a lower fluorescence were a small fraction of total wells on chip and confined to the chip boundaries as shown in Supplementary Fig. 1e (scale bar: g 500 µm; h 100 µm)
