Fig. 4
From: Pixelated spatial gene expression analysis from tissue
On-chip RT-LAMP for TOP2A mRNA. a Raw fluorescence images of real-time RT-LAMP with tissue on chip at four different time points (*Tissue, **No tissue) (scale bar: 200 µm). b Fluorescence bar graphs of the raw images showing a differential increase in fluorescence over time. The gain in fluorescence over time is calculated taking time = 0 image (initial) as the reference. c Spatial threshold analysis showing the spatially mapped threshold times. Note that the tissue boundaries are maintained throughout the reaction. Each pixel is 100 µm × 100 µm. d Raw amplification curves of a row marked in red in “c” showing positive and negative wells. e Fluorescence curves for all wells after curve fitting
