Fig. 5
From: Pixelated spatial gene expression analysis from tissue
On-chip RT-LAMP: cancer vs. non-cancer control. a Raw fluorescence images of real-time RT-LAMP with prostate cancer tissue on right and non-cancer (mouse skeletal muscle) tissue on left of chip at four different time points (*Non-cancer, **Cancer) (scale bar: 200 µm). b Fluorescence bar graphs of the raw images showing a differential increase in fluorescence over time. The gain in fluorescence over time is calculated taking time = 0 image (initial) as the reference. Note the amplification occurs only for the cancerous tissue. c Spatial threshold analysis showing the spatially mapped threshold times. Each pixel is 100 µm × 100 µm. d Raw amplification curves of a row showing positive and negative wells marked in red in “c”. e Fluorescence curves for all wells after curve fitting
