Fig. 6
From: Pixelated spatial gene expression analysis from tissue
On-chip RT-LAMP with mRNA FISH on serial sections. a Baseline-subtracted fluorescence images of real-time RT-LAMP with tissue on chip at three different time points showing the increase in fluorescence over time (scale bar: 200 µm). b Spatial threshold analysis showing the spatially mapped threshold times. No amplification was observed till 36 min. c DAPI (blue) and TOP2A mRNA FISH (red) images of the consecutive section showing spatial heterogeneity in TOP2A mRNA expression (scale bar: 200 µm). d Pixelated intensity map of mRNA FISH fluorescence. The spatial pattern of TOP2A expression is similar between the two assay types. For b, d, each pixel is 100 µm × 100 µm
