Fig. 3
From: Cell fate in antiviral response arises in the crosstalk of IRF, NF-κB and JAK/STAT pathways
Transcriptional activation and secretion of IFNβ. a Time profiles of Ifnb1 (INFβ) mRNA levels in response to different stimuli. WT MEFs were stimulated with 1000 U/ml IFNβ, 1 μg/ml LPS, or 1 μg/ml poly(I:C) in the absence (left) or presence (right) of IFNAR-blocking antibody (α-IFNAR). Stat1–/– MEFs and RelA–/– MEFs (right) were also stimulated with 1 μg/ml poly(I:C). α-IFNAR was added to cells at 10 μg/ml at 0, 3, 6 and 10 h after poly(I:C) transfection. Time profiles of relative mRNA levels were obtained with RT-PCR, and then rescaled to absolute numbers using digital PCR measurements (bars represent means ± s.e.m., n ≥ 2; see Supplementary Note for plots of all replicates compared with model simulations). b Secretion of IFNβ in response to TNFα, LPS or poly(I:C). WT MEFs were stimulated with 10 ng/ml TNFα (n = 2), 1 μg/ml LPS, 0.1, 0.3, 1 and 3 μg/ml poly(I:C), or 1 μg/ml poly(I:C) in the presence of α-IFNAR (n = 2). Both Stat1–/– MEFs and RelA–/– MEFs were stimulated with 1 μg/ml or 3 μg/ml poly(I:C). For each condition, IFNβ concentration after 2, 4, 6, 10 and 24 h was measured by ELISA in 200 μl of culture medium harvested from above 25,000 ± 5000 cells. Bars represent means ± s.e.m. (n = 3, except where stated otherwise, values of all replicates are provided in Supplementary Table 8)
