Fig. 8
The effect of caspase-1 inhibitor on ZIKV-induced inflammatory responses in mice. A129 mice (6 weeks old) were treated with DMSO or Ac-YVAD-cmk (8 mg/kg) by intraperitoneal injection 30 min and infected with ZIKV (5 × 105 PFU) or treated with PBS for the indicated times. a, b A129 mice were pretreated with DMSO (n = 3; 2 males and 1 female) (a) or Ac-YVAD-cmk (n = 3; 2 males and 1 female) (b) and then infected with ZIKV (5 × 105 PFU) for 0, 2, 4, and 6 days. ZIKV RNA in the mice blood was determined by RT-PCR. Data shown are whiskers: Min.–max.; *P < 0.05, **P < 0.01, ***P < 0.0001 (one-way ANOVA with Tukey’s post-hoc test). c–f A129 mice pretreated with DMSO (n = 3; 2 males and 1 female) or Ac-YVAD-cmk (n = 3; 2 males and 1 female) were infected with ZIKV (5 × 105 PFU) for 11 days. The mice brain, spleen, liver, and kidney tissue sections were stained with DAPI to label nuclei (blue) and an antibody against ZIKV E protein (red) and then examined under confocal microscopy. Scale bar is 100 μm. g A129 mice pretreated with DMSO (n = 3; 2 males and 1 female) or Ac-YVAD-cmk (n = 4; 2 males and 2 females) were infected with ZIKV (5 × 105 PFU) for 0, 2, 4, and 6 days. IL-1β levels in mice sera were determined by ELISA. Data shown are means ± s.e.m. h–k A129 mice pretreated with DMSO (n = 3; 2 males and 1 female) or Ac-YVAD-cmk (n = 4; 2 males and 2 females) were infected with ZIKV (5 × 105 PFU). At 11 days posttreatment, the mice brain, spleen, liver, and kidney tissue samples were stained with IL-1β antibody (h) or stained with hematoxylin and eosin (H&E) (i) and then examined using a light microscope. At 0, 2, 4, 6, 7, 8, 9, 10, 11, 12, and 13 days posttreatment, the mice body weights (j) and survival rates (k) were evaluated. Scale bar is 100 μm (h, i). Data shown are means ± s.e.m (j)
