Fig. 6
Histone crotonylation is cell cycle regulated by class I HDACs. Cell cycle block and release experiment on HCT116 cells using CDK4/6 inhibitor abemaciclib with and without MS275. Lanes 1 and 9: asynchronous cells, lane 2: G1 arrested cells, lanes 3–8: increase in histone crotonylation (Kcr), H3K18cr and H3K18ac upon release into S phase. Lanes 10–16: histone crotonylation, H3K18cr and H3K18ac are upregulated during a G1 arrest and S phase when class I HDACs are inhibited with MS275. For the experiments in lanes 9–16, cells were blocked in G1 using 15 nM abemaciclib in the presence of 5 μM MS275 and released into S phase in the presence of 1 μM MS275. Cell cycle profiles are shown at the top, western blots in the middle, and quantifications of those, as calculated relative to H3 and normalized to the DMSO (vehicle) sample, at the bottom. Representative of two experiments is shown
