Fig. 6
From: Active and dynamic mitochondrial S-depalmitoylation revealed by targeted fluorescent probes
MitoDPP-3 reveals APT1 activity in mitochondria. a HEK293T cells treated for 30 min with 1 µM Hoechst 33342, 100 nM MitoTracker Deep Red, and either DMSO or 10 µM PalmB for 30 min, washed, loaded with 324 nM mitoDPP-3 for 10 min, and then analyzed by confocal fluorescence microscopy. 5 µm scale bars shown. b Enlarged portion of image indicated by dotted white box in (a) showing colocalization of mitoDPP-3 with MitoTracker. Quantification of the relative fluorescence intensity from mitoDPP-3 and MitoTracker in either control or PalmB-treated cells (c). ML348-treated cells (d), ML349-treated cells (e), cells with APT1 knocked down (f), or cells with APT2 knocked down (g). For all plots, statistical analyses performed with a two-tailed Student′s t-test with unequal variance, *P value < 0.05; **P value < 0.009, n = 3 for (c), n ≥ 4 for (d–g), error bars are ± s.e.m
