Fig. 1 | Nature Communications

Fig. 1

From: Correspondence: Reply to ‘Compound 17b and formyl peptide receptor biased agonism in relation to cardioprotective effects in ischaemia-reperfusion injury’

Fig. 1

Signalling fingerprint of R,S-(±)Cmpd17b and its enantiomers in CHO cells stably expressing hFPRs. Influence of R,S-(±)Cmpd17b (blue), R-(−)Cmpd17b (green) and S-(+)Cmpd17b (purple) on intracellular signalling intermediates downstream of GPCRs. Concentration–response curves to Ca2+i mobilisation at a hFPR1 and b hFPR2. Concentration–response curves to ERK1/2 phosphorylation at c hFPR1 and d hFPR2. Responses to all three compounds were determined concomitantly, with n = 3–7 separate experiments, each performed in duplicate or triplicate. Results are expressed as mean ± S.E.M. The red dashed line illustrates non-linear regression for the reference FPR agonist Cmpd43 from our original publication6. The chemical structure of Cmpd17b (N-(4-bromophenyl)-2-[5-(3-methoxybenzyl)-3-methyl-6-oxo-6H-pyridazin-1-yl]-propionamide), illustrating the position of the chiral centre, is shown on the upper panel

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