Fig. 2
From: Lack of beta-arrestin signaling in the absence of active G proteins
G protein activation and βarr2 recruitment in absence and presence of active G proteins. a Inhibition of forskolin-mediated cAMP accumulation by C3-activated FFA2 in FFA2-HEK293 cells, in the presence or absence of PTX. b IP1 accumulation of C3-stimulated FFA2, in the presence or absence of FR. c, d FRET measurements of Gi (c) and Gq (d) activation-induced conformational changes in wild-type (WT) HEK293 cells expressing FFA2 after stimulation with C3 in absence and presence of G protein inhibitors. e, f C3-induced β-arrestin2 recruitment to FFA2 in wild-type (WT) (e) or ΔG12/13 HEK293 cells (f), in the absence and presence of PTX and FR. Shown are representative FRET traces with the indicated number of total cells per condition (c, d) and bar diagrams are averages from three independent experiments. Data are mean ± s.e.m. (a, b, e, f) or + s.d. (c, d) of three experiments each performed in triplicate. For statistical analysis, two-tailed unpaired t-test (c, d) and two-sample paired Wilcoxon test (e, f) was applied to paired points at different concentrations. *P < 0.05; **P < 0.01; ***P < 0.001; not significant where no asterisk
