Fig. 4
From: Lack of beta-arrestin signaling in the absence of active G proteins
G protein vs. βarr1/2 dependence of ligand-stimulated ERK1/2 phosphorylation. a–c Kinetic pERK1/2 and total ERK1/2 profile of PGD2-stimulated DP2 (a), MDL-activated GPR17 (b), and C3-stimulated FFA2 (c) in the absence and presence of PTX and FR in wild-type (WT) HEK293 cells. d–f Temporal pattern of pERK1/2 and total ERK1/2 for PGD2-stimulated DP2 (d), MDL-stimulated GPR17 (e), and C3-stimulated FFA2 (f) in Δβarr1/2 cells in the absence and presence of PTX and FR. Data are mean +/± s.e.m. of three (b, c, f) and four (a, d, e) independent experiments (3 technical replicates each). For statistical analysis, one-sample t-test (b) was performed. **P < 0.01
