Fig. 5
From: SWELL1 is a glucose sensor regulating β-cell excitability and systemic glycaemia
SWELL1-mediated ICl,SWELL is required for glucose-stimulated Ca2+ signaling in primary β-cells. a Ad-RIP1-GCaMP6s-transduced Swell1fl/fl murine islet. Scale bar represents 50 µm. b–d GCaMP6s Ca2+ transients in WT (b, Ad-RIP1-GCaMP6s/Swell1fl/fl) and SWELL1 KO (c, Ad-RIP1-Cre-P2A-GCaMP6s/Swell1fl/fl) primary murine β-cells in response to 16.7 mM glucose stimulation (basal 1 mM glucose) and 40 mM KCl stimulation. Insets show β-cell fluorescence images at the indicated times in the experiment. d Mean peak values of GCaMP6s Ca2+ transients from Ad-RIP1-GCaMP6s/Swell1fl/fl (n = 14 cells) and Ad-RIP1-Cre-P2A-GCaMP6s/Swell1fl/fl (n = 10 cells). e Ad-RIP1-GCaMP6s-transduced human islet. Scale bar represents 50 µm. f–h GCaMP6s Ca2+ transients in human primary β-cells co-transduced with Ad-RIP1-GCaMP6s+Ad-shSCR-mCherry (f) and Ad-RIP1-GCaMP6s+Ad-shSwell1-mCherry (g) in response to 16.7 mM glucose stimulation (basal 1 mM glucose) and 40 mM KCl stimulation. h Mean peak values of GCaMP6s Ca2+ transients from shSCR (n = 6 cells) and shSwell1 (n = 8 cells) transduced human β-cells. Data are shown as mean ± s.e.m. One-way ANOVA for in-group comparison, unpaired t-test for between-group comparison. *p < 0.05, **p < 0.01, ***p < 0.001. ns, not significant
