Fig. 7
From: SWELL1 is a glucose sensor regulating β-cell excitability and systemic glycaemia
β-cell SWELL1 is required for glucose-stimulated insulin secretion in vitro. a Glucose-stimulated insulin secretion (GSIS) in WT and Swell1 KO MIN6 cells in response to 1, 5.5, and 30 mM glucose (n = 3, each). b Total insulin content normalized to total MIN6 cell protein content (n = 3, each). c Representative image of Ad-RIP1-Cre-RFP-transduced mouse islet. Scale bar represents 50 µm. d GSIS in WT (Ad-RIP1-RFP/Swell1fl/fl) and β-cell-targeted SWELL1 KO (Ad-RIP1-Cre-RFP/Swell1fl/fl) islets. Insulin secretion is expressed as percentage of the total insulin content (n = 4, each). e Total insulin content of WT and β-cell-targeted Swell1 KO islets (n = 4, each). f GSIS in human WT and SWELL1 KD islets. Insulin secretion is expressed as percentage of the total insulin content (n = 4, each). g Total insulin content of WT and SWELL1 KD islets (n = 4, each). Data are shown as mean ± s.e.m. One-way ANOVA for in-group comparison, unpaired t-test for between-group comparison. *p < 0.05, **p < 0.01, ***p < 0.001. ns, not significant
