Fig. 1
Self-hematopoietic antigen H60 derives partial negative selection of J15 thymocytes. a Flow cytometric analysis of thymocytes from F1 progenies of crossing J15 mice with B6, Con-H60, and Act-H60 mice. Representative CD4-phycoerythrin.Cychrome 5 (PE.Cy5) by CD8-allophycocyanin.Cychrome 7 (APC.Cy7) FACS profiles are shown with gating on Vβ8.3+ cells. H60-tetramer-PE by CD8-APC.Cy7 FACS profiles are shown after further gating on CD8+ SP cells. The percentages are indicated in the FACS profiles. The number of total thymocytes; percentages of CD4−CD8−DN, CD4+CD8+ DP, and CD8+ SP cells in the total thymocytes; and percentages and MFI values of H60-tetramer+ cells in CD8+ SP cells were plotted. b Analysis of splenocytes from the three different J15 F1 mice. The numbers of splenic CD8+ T cells and the frequencies and MFI values of H60-tetramer-binding cells among the splenic CD8 T cells are plotted. Representative FACS data show H60-tetramer-PE staining in splenic CD8 T cells after gating on Vβ8.3+ cells; the percentages of H60-tetramer-binding cells are indicated. All data (a, b) represent more than three independent experiments (n = 3/group/experiment) and are presented as means ± s.e.m. P-values were generated by Student’s t-test
