Fig. 2
gp100-positive cells from SLNs and primary tumours display multiple copy number alterations (CNAs). a Ninety-one DCCs from our patients were selected according to QC criteria (see main text and Supplementary Figure 1) for CGH and mutation analysis. Histograms depict the genomic gains (yellow) or losses (blue) per cell. The identifiers indicate cell ID. b Cluster analysis of paired PTs (n = 23 samples) and DCCs (n = 24 samples) from 19 patients for chromosomal aberrations (gain = +1; loss = −1). Only the 10 most variable regions are included (see Supplementary Figure 5 for all 53 variable regions). Side-line identifiers indicate patient ID, sample type (PT and DCC) and sample index; bottom-line labels indicate chromosomal regions. Black (28T), white (30T) and dark grey (09T) filled squares indicate examples of PT-DCC pairs for which several areas of the PT were available. Examples of paired DCCs and PTs with varying PT thickness are indicated by coloured squares (green: 1 mm, grey: 1.4 mm, pink: 2 mm, blue: 7 mm PT thickness). c Comparison of paired PTs and DCCs for chromosomal aberrations. Displayed are the 18 chromosomal regions that differ significantly (FDR-adjusted Fisher’s exact test p value ≤0.05) between paired PTs (n = 23, 19 patients) and DCCs (n = 24, n = 19 patients) regarding aberration frequency. Gains and losses are given in percent. d Maximum number of DCC samples NDCC (colour-coded) that cannot be excluded to derive from corresponding DCC-like clones within the PT plotted against the assumed DCC-like clone fraction in the PT and the successful dissemination fold change. If DCC-like and non-DCC-like PT clones disseminate equally (fold = 1), at most 11/24 DCC samples may result from DCC clones within the PT at the maximum indicated clone fraction of 0.40 (A); for twofold dissemination not more than 14/24 (B). For fold = 5, the same number of samples can be excluded up to a fraction of 0.124 (11/24) (C) and 0.21 (14/24) (D), respectively. These intermediate fractions correspond to a maximum of 5/24 (0.124) (E) and 7/24 (0.21) (F) DCC samples, respectively, if clones disseminate similarly (fold = 1). See 'Methods' section for details. Significance level α = 0.05 (Poisson-binomial distribution)
