Fig. 3

HIF-1α deficiency impairs CD1d^hiCD5⁺ B cells IL-10 production and expansion. a Representative plots and quantification of IL-10-producing B cells in bone marrow (BM), spleen (SP), inguinal lymph nodes (ILN), and peritoneal cavity (PerC) from Mb1^creHif1a^f/f, Mb1^creHif2a^f/f, and control mice (n = 3 per group). b Representative plots and absolute numbers of CD19⁺B220⁺CD1d^hiCD5⁺ B cells in spleen from Mb1^creHif1a^f/f (n = 6) and WT mice (n = 6). c Percentage of BrdU⁺ cells in CD1d^hiCD5⁺, CD1d^loCD5⁻, or total splenic B cells isolated from WT (n = 6) and Mb1^creHif1a^f/f mice (n = 6) 7 days after BrdU treatment. d Representative plots and quantification of IL-10⁺CD1d^hiCD5⁺ B cells in spleen from Mb1^creHif1a^f/f (n = 7) and WT mice (n = 7). e IL-10 production by sorted CD1d^hiCD5⁺ B cells from Mb1^creHif1a^f/f (n = 5, 6) and WT mice (n = 5, 6) after stimulation with LPS (left) or anti-IgM (right) for 48 h. Data are shown as mean ± s.e.m. Pictures are representative of three independent experiments. NS not significant; *P < 0.05, **P < 0.01, and ***P < 0.001 (two-tailed unpaired Student’s t-test) (see also Supplementary Figure 3)