Fig. 2

DOT1L mutants in human melanoma are loss of function. a In vitro H3K79me2 levels from melanoma cells with known DOT1L mutation status from Queensland, Australia. b DOT1L-depleted HPMs were infected with lentivirus encoding WT or mutant DOT1L as indicated. MV411 cells were transiently transfected with WT or mutant DOT1L as indicated. c, d MV411 cells or DOT1L-depleted HPMs with WT or mutant DOT1L overexpression were treated with EPZ-5676 (5 µM). Cell growth was detected using cell number counting (c) or MTT assay (d). e H3K79me2 signal intensities in the gene body regions, under the condition of C021 shControl, C021 shDOT1L, and C025 shControl. *p < 0.05, **p < 0.01, ***p < 0.001, unpaired Student’s t-test. Error bars represent ± s.d.