Fig. 4
From: The protective role of DOT1L in UV-induced melanomagenesis
UVR-induced melanoma development in melanocyte-specific DOT1L conditional knockout mice. a Experimental design for UVR-induced melanoma development in the DOT1Lflox/flox/Tyr-CreERT2 mice. b Melanoma incidence in wild-type and DOT1Lflox/flox/Tyr-CreERT2 mice with or without UVB irradiation. c Image of a representative DOT1Lflox/flox/Tyr-CreERT2 cutaneous melanoma. d H&E staining of a representative cutaneous melanoma. Scale bar, 500 μm (upper panel) and 50 μm (lower panel). e Immunohistochemistry staining of S100, tyrosinase, Pax3, and Mart-1 of the representative cutaneous melanoma. Scale bar, 100 μm. f The relative mRNA expression of Pax3 and tyrosinase in mouse melanomas comparing to those of normal skin tissues. ***p < 0.001, unpaired Student’s t-test. Error bars represent ± s.d.
